The b fragment peaks are labeled from the amino to the carboxyl terminus De Novo sequencing seems petty simple right? Because this equation describes a line in terms of its slope and its y-intercept, this equation is called the
The calculated masses are shown above each y ion in bold numbers Below in Figure 5 the first six y ions are illustrated in some detail

## Magnetic Field Strength

It looks like you wrote the slope as instead of.

De Novo Peptide Sequencing
A positive y-intercept means the line crosses the y-axis above the origin, while a negative y-intercept means that the line crosses below the origin
De Novo Peptide Sequencing
The fragment containing only the amino terminal amino acid is termed b1
Connecting the Coast
The bar labeled b changes the y-intercept
Advanced Question Which of the following lines goes through the points and? Can you still write the equation? Similarly, groups of peptide fragment ions appear to extend from the C-terminus, these peaks are termed, " y ions" Check the + and — signs in the problem again
But when the generated fields pass through which themselves contribute internal magnetic fields, ambiguities can arise about what part of the field comes from the external currents and what comes from the material itself The nomenclature is very simple to follow

## De Novo Peptide Sequencing

So, if you have a point on the line, it must be a solution to the equation.

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Magnetic Field Strength
To find the slope of the line, use the formula
Magnetic Field Strength
In this equation, x and y are coordinates of a point, m is the , and b is the y-coordinate of the
De Novo Peptide Sequencing
Recall that a point is an x, y coordinate pair and that all points on the line will satisfy the linear equation
Changing the value of b moved the line around the coordinate plane When you find the slope, use it and one of the given points to find the value of b
It is more challenging, but you can find the equation of the line that would pass through those two points Substitute the slope m into

## Writing the Equation of a Line

To find b, substitute the known values for x, y, and m into the slope-intercept form.

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Connecting the Coast
The slope-intercept form can also help you to write the equation of a line when you know the slope and a point on the line or when you know two points on the line
De Novo Peptide Sequencing
The fragment containing the first two amino terminal amino acids is termed the b2 ion, and so forth
Writing the Equation of a Line
Find the slope using the given points